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Biomolecular adsorption has great significance in medical, environmental, and technological processes. Understanding adsorption equilibrium and binding kinetics is essential for advanced process implementation. This requires identifying intrinsic determinants that predict optimal adsorption properties at bio–hybrid interfaces. Solid-binding peptides (SBPs) have targetable intrinsic properties involving peptide–peptide and peptide–solid interactions, which result in high-affinity material-selective binding. Atomic force microscopy investigations confirmed this complex interplay of multi-step peptide assemblies in a cooperative modus. Yet, most studies report adsorption properties of SBPs using non-cooperative or single-step adsorption models. Using non-cooperative kinetic models for predicting cooperative self-assembly behavior creates an oversimplified view of peptide adsorption, restricting implementing SBPs beyond their current use. To address these limitations and provide insight into surface-level events during self-assembly, a novel method, the Frequency Response Cooperativity model, was developed. This model iteratively fits adsorption data through spectral analysis of several time-dependent kinetic parameters. The model, applied to a widely used gold-binding peptide data obtained using a quartz crystal microbalance with dissipation, verified multi-step assembly. Peak deconvolution of spectral plots revealed distinct differences in the size and distribution of the kinetic rates present during adsorption across the concentrations. This approach provides new fundamental insights into the intricate dynamics of self-assembly of biomolecules on surfaces. 

The forest floor of Watershed 6 was first sampled in 1969-70. These data include forest floor thickness, soil mass, organic matter content, and major-element composition for samples collected since 1976. Watershed 6 has been resampled at intervals varying from one to ten years. Sampling at five to ten year intervals is expected to continue. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station. 

We sampled soils on watershed 5 at the Hubbard Brook Experimental Forest in 1983, prior to a whole-tree harvest conducted in the winter of 1983-84. We resampled in 1986, 1991, and 1998. All sampling was performed using a quantitative soil pit method. Samples of the combined Oi and Oe horizons; the Oa horizon; 0-10 cm, 10-20 cm, and >20 cm layers of mineral soil; and the C horizon were collected. Grab samples of pedogenic mineral horizons were also taken from the sides of a subset of pits in each year. Here we report soil chemistry, mass of soil, percent rock, bulk density, and organic matter. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.  

{"Abstract":["This data set includes chemistry of O-horizons ("forest floor") and the 0-10 cm \nmineral soil layer in Watershed 1 at Hubbard Book. Calcium in the form of wollastonite \n(CaSiO3) was added to Watershed 1 in October 1999. The application rate was 1028 kg \nCa per ha, and the application was relatively uniform across the watershed. Pre-treatment \nforest floor surveys were completed in 1996 and 1998. The first post-treatment forest \nfloor survey was completed in 2000. This data set includes mass and thickness data for \nthe sampled layers. Chemical data include concentrations and pools of organic matter, \nC, N, Ca, Mg, K, P, Mn, Fe, Al, Cu, Pb, and Zn. Soil pH and exchangeable Al, Ca, Mg, K, \nand H are also included. Sampling is intended to continue at 4 or 5 year intervals.\n These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). \nThe HBES is a collaborative effort at the Hubbard Brook Experimental Forest, \nwhich is operated and maintained by the USDA Forest Service, Northern Research Station."]} 

The forest floor of Watershed 6 was first sampled in 1969-70. These data include forest floor thickness, soil mass, organic matter content, and major-element composition for samples collected since 1976. Watershed 6 has been resampled at intervals varying from one to ten years. Sampling at five to ten year intervals is expected to continue. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station. 

In 1990-1991 segments of boles from felled sugar maple (Acer saccharum), yellow birch (Betula alleghaniensis) and American beech (Fagus grandifolia) trees were placed in the field to study the rate of decomposition and nutrient loss (or gain) over time. The segments incubated in the field, ranging from 0.5-1.3 meters in length, were paired with fresh segments from the same trees. The fresh segments were taken to the lab shortly after felling, dried, weighed and subsampled. Fresh samples of wood and bark were collected separately. Incubated bole segments were collected in 1993 (T1), 1997 (T2), 2001 (T3), 2007 (T4) and 2015/2016 (T5). The whole bole segments were transported to the lab, measured, dried and weighed to determine mass loss. Subsamples of the bole wood and bark were collected for chemical analysis, including C, N, H, Ca, Mg, K, Si, Al, Pb, Zn, Mn and Fe. Chemical analyses were conducted concurrently on the fresh (T0) and incubated samples. This data set includes the masses of the fresh and incubated boles along with the concentrations of the chemical analytes. Element pools in the boles can be calculated by multiplying the concentrations by the mass values. This data set includes chemical data for samples collected in 1993, 2001, and 2007 and their paired fresh samples. Samples from 1997 were measured for mass, but inadvertently discarded prior to chemical analysis. These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.